Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 23;10:3830. doi: 10.1038/s41467-019-11739-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — Brain regions are differentially recruited during epileptic activity. a An optical section of a zebrafish larva expressing GCaMP6s in all neurons, obtained by two-photon microscopy, dorsal view (left). Individual neurons (right) in color-coded brain regions: telencephalon (dark blue), thalamus (light blue), optic tectum (green), cerebellum (yellow), and brainstem (orange). b Activity of individual neurons (ΔF/F) over time, organized by brain region. White dashed line indicates the start of 20 mM pentylenetetrazole (PTZ) perfusion. Warmer colors indicate stronger activity. c, d Percentage of active neurons (>3std_baseline) in the whole brain (c), and per brain area (d) during baseline (black), preictal (gray) and seizure periods (red). e, f Average activity of the active neurons, defined by the area under the curve of the ΔF/F trace, in the whole brain (e) and per brain area (f). g Confocal image of Tg(gad1:GFP);Tg(vglut2a:dsRED) double transgenic zebrafish larva showing glutamatergic (magenta) and GABAergic (green) neurons. White bars reflect 100 µm. **p = < 0.01, *p = < 0.05, ns = not significant, Wilcoxon signed-rank test. Error bars (c-f) represent the s.e.m. of n = 8 fish, where 2012.6 ± 238.7 (mean ± SEM) neurons/fish were analyzed