Table 4.

Primer sequences for chitin synthase 1 (CHS1) and internal control genes used to determine the expression profile in A. gossypii following exposure to buprofezin.

Primer name	Sequence (5′-3′)	Application
CHS1-F	ATTGCGTCACGATGATCCTT	qRT-PCR
CHS1-R	TGGTCGCTAGACGTTCACAC	qRT-PCR
EF1α-F	GAAGCCTGGTATGGTTGTCGT	qRT-PCR
EF1α-R	GGGTGGGTTGTTCTTTGTG	qRT-PCR
β-Actin-F	GGGAGTCATGGTTGGTATGG	qRT-PCR
β-Actin-R	TCCATATCGTCCCAGTTGGT	qRT-PCR
GAPDH-F	AACAGTTTTTTGAGTGGCGGT	qRT-PCR
GAPDH-R	TGGTGTCAACTTGGATGCGTA	qRT-PCR