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. 2019 Aug 23;10:3814. doi: 10.1038/s41467-019-11772-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Conformational response of CSN5/CSN6 to NEDD8. Differential HDX-MS of a Δ(CSN–CRL2~N8 - CSN) and b Δ(CSN^WT–CRL2 - CSN^WT). Regions exhibiting significant deuterium uptake differences in (i) CSN6, (ii) CSN5 and (iii) CSN5 active site are highlighted in red for destabilised and blue for stabilised areas. Regions without coverage or exhibit non-significant changes are in grey and white, respectively. c–d Deuteration profiles of CSN5 active site (as shown in a–b iii). Profiles of the c non-neddylated and d neddylated holocomplex (red) are compared with deuteration profiles of the isolated CSN (blue). The ion distribution of active site peptide is shown for c and d across reference 0 s, 15 s, 5 m and 30 m timepoints. The mass of the non-deuterated reference peptide is shown by the dotted line. A second dotted line for the holocomplexes (red) indicates the mass of the peptide at the 30 m timepoint. The relative deuterium changes of the active site peptide in c following binding of CRL2 is negligible, while in d, presence of CRL2~N8 leads to a significant increase in mass. Source Data are provided as a Source Data file