Table 1.
Cryo-EM data collection, refinement and validation statistics
| CSN–CRL2~N8 (holocomplex) (EMB-4739) (PDB 6R7F) | CSN−CSN5/CSN6-CRL2−VHL~N8 (EMB-4744) (PDB 6R7N) | CSN–CRL2−VHL~N8 (CSN5/CSN6 refined) (EMB-4742) (PDB 6R7I) | CSN–CRL2~N8 (VHL refined) (EMB-4736) (PDB 6R6H) | CSN–CRL2 (EMB-4741) (PDB 6R7H) | |
|---|---|---|---|---|---|
| Data collection and processing | |||||
| Magnification | 47,170 | 47,170 | 47,170 | 47,170 | 47,755 |
| Voltage (kV) | 300 | 300 | 300 | 300 | 300 |
| Electron exposure (e-/Å−2) | 45 | 45 | 45 | 45 | 83 |
| Defocus range (μM) | 1.8–3.0 | 1.8–3.0 | 1.8–3.0 | 1.8–3.0 | 1.8–3.0 |
| Pixel size (Å) | 1.060 | 1.060 | 1.060 | 1.060 | 1.047 |
| Symmetry imposed | C1 | C1 | C1 | C1 | C1 |
| Initial number of particles | 316,921 | 316,921 | 316,921 | 316,921 | 308,936 |
| Final number of particles | 20,055 | 22,471 | 24,552 | 24,049 | 17,191 |
| Map resolution (Å) | 8.2 | 6.5 | 8.0 | 8.4 | 8.8 |
| FSC threshold | 0.143 | 0.143 | 0.143 | 0.143 | 0.143 |
| Map resolution range (Å) | 6.7–22.7 | 5.9–8.0 | 5.9–11.5 | 6.3–17.4 | 5.6–16.8 |
| Refinement | |||||
| Subunits missing | None | CSN5/CSN6, VHL | VHL | None | None |
| Initial PDB used | 4D10, 5N4W, 4WQO, 3DQV | 4D10, 5N4W, 4WQO | 4D10, 5N4W, 4WQO | 4D10, 5N4W, 4WQO | 4D10, 5N4W, 4WQO |
| Number of residues | 3727 | 2974 | 3578 | 3716 | 3251 |