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. 2019 Aug 23;9:12320. doi: 10.1038/s41598-019-48795-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of PGC-1α in skeletal muscle does not influence number of immature or mature DCX⁺-cells in the DG of middle-aged animals. Image showing DCX⁺ immunostainings of DG for 11-month-old animals demonstrating (a) parallel and perpendicular orientation, with corresponding graphs showing number of DCX⁺ cells with (b) parallel and (c) perpendicular orientation (two-way ANOVA; n = 7–9; n.s.) for 11-month-old animals. Data expressed as mean ± SEM for female and male WT and MCK-PGC-1α animals. Scale bars = 50 µm.