Fig. 4.

Immunogold localization of PKHD1L1 towards the tips of OHC and IHC stereocilia (P4). a, b SEM photomicrographs of P4 OHC stereocilia bundles. Multiple 12-nm gold beads in a (yellow arrows point to some gold beads) were detected on the surface of control Pkhd1l1^fl/fl, Atoh1-Cre⁻ OHCs, but not mutant Pkhd1l1^fl/fl, Atoh1-Cre⁺ hair cells, further validating the antibody specificity at the EM level in b (yellow arrow shows nonspecific label). c–h 10-nm gold beads were detected on TEM photomicrographs. Black arrows point to gold beads on the surface of IHC stereocilia c, OHC kinocilia d, and stereocilia e–h; black arrowheads point to the tip links c, e, f. Scale bars: a–b, 1 µm; c–h, 200 nm