Fig. 2.

Large-scale nucleic acid photolithography (Level-One patterning). Rendering in DNA of a color image. Original RGB is split into individual color channels, each of which is reduced to 8 shades of gray. Each shade of each color channel is assigned a DNA sequence with a calibrated melting temperature with a fluorescently-labeled complementary oligonucleotide. A sequence for each pixel is constructed with one of the resulting 512 variants. After photolithographic synthesis of all the DNA sequences, the surface is hybridized with the three complementary or partially complementary Cy3-, Cy5- or fluorescein-labeled oligonucleotides. The scale bars in the resulting fluorescence images are 200 µm