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. 2019 Aug 23;9:12317. doi: 10.1038/s41598-019-48566-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in decellularized matrix topography and mechanics assessed in Amnion. (A) Height and PeakForce error images of the bare matrix show the presence of very thin and thick irregular fibres in bare matrix. (B) After seeding and culture of normal, scar and Dupuytren’s fibroblasts for two weeks, height and PeakForce error images show the presence of fibroblasts on Amnion matrix. (C) After removing the cells, height and PeakForce error images show the disappearance of fibres. Histograms of Young’s modulus values of bare (black bar), cell-occupied (blue bar), and cell- removed Amnion matrix (red bar), for normal (D), scar (E) and Dupuytren’s fibroblasts (F). The shifts in the histograms show the change in Amnion matrix mechanics effected by all three fibroblast types. The respective medians of Young’s modulus values are shown in Supplementary Fig. 2A. Filled and open arrow heads indicates the thick and thin fibres, respectively.