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. 2019 Aug 23;9:12317. doi: 10.1038/s41598-019-48566-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in decellularized matix topography and mechanics assessed in Epiflex. (A) Height and PeakForce error images of bare matrix show the presence of bigger blobs with some very thin fibres in the bare matrix. (B) After culture the three fibroblast types for two weeks, height and PeakForce error images show the topography of matrix with respective cells. (C) After removing the cells, height and PeakForce error images shows the change in matrix topography. Histograms of Young’s modulus values of bare (black bar), cell-occupied (blue bar), and cell-removed Epiflex matrix (red bar), for normal (D), scar (E) and Dupuytren’s fibroblasts (F). The shifts in the histograms show the change in Epiflex matrix mechanics by all three fibroblast types. The respective medians of the Young’s modulus values are shown in Supplementary Fig. 2C. Open arrow heads indicates the thin fibres.