Table 1.
Summary of cell-type-specific H3K4me3 ChIP-Seq
| Substage | Experiment | Antibody | Purity (%) | Starting chromatin DNA (ng) | SPoT (HS; %) | SPoT (All; %) | Peaks (#) |
|---|---|---|---|---|---|---|---|
| Leptonema | 1 | EpiCypher | 96.9 | 2398 | 2.4 | 4.5 | 9528 |
| Leptonema | 2 | EpiCypher | 95.3 | 805 | 2.2 | 7.6 | 13,095 |
| Leptonema | 2 | Abcam | 95.3 | 805 | 3.3 | 20.3 | 30,943 |
| Zygonema | 1 | EpiCypher | 79.7 | 2688 | 4.7 | 6.4 | 15,699 |
| Zygonema | 2 | EpiCypher | 64.6 | 755 | 3.6 | 4.2 | 11,314 |
| Zygonema | 2 | Abcam | 64.6 | 755 | 5.3 | 17.0 | 29,114 |
| Early Pachynema | 1 | EpiCypher | 81.6 | 1793 | 1.6 | 4.2 | 6647 |
| Early Pachynema | 2 | EpiCypher | 89.5 | 4585 | 1.7 | 2.3 | 4883 |
| Early Pachynema | 2 | Abcam | 85.2 | 960 | 2.0 | 14.0 | 17,561 |
| Late Pachynema | 1 | EpiCypher | 84.1 | 4936 | 1.7 | 2.6 | 5189 |
| Late Pachynema | 2 | EpiCypher | 71.3 | 915 | 1.6 | 3.8 | 9124 |
| Late Pachynema | 2 | Abcam | 71.3 | 915 | 1.5 | 20.1 | 19,711 |
| Diplonema | 1 | EpiCypher | 88.7 | 6502 | 1.8 | 3.1 | 6645 |
| Diplonema | 2 | EpiCypher | 82.3 | 1115 | 1.7 | 5.4 | 12,071 |
| Diplonema | 2 | Abcam | 82.3 | 1115 | 1.6 | 21.0 | 18,191 |