Fig. 7.

Pip and G3P cooperate to promote SAR and PTP cues. a Experimental setup of (b–d). Senders were exposed to Pin, treated with Pip and/or G3P, or exposed to the appropriate mock controls, and incubated with naive receivers in gas-tight containers. The receivers were subsequently released from the containers and inoculated with Pst. b, c In planta Pst titers at 4 dpi of the receivers. The plant genotypes are indicated below the panels (senders in purple and receivers in green) and the treatments of the senders are indicated below the bars. d Chemical complementation of the PTP-signaling defect of the ald1 mutant with Pip and G3P. ald1 senders were irrigated with Pip or the corresponding H₂O control and subsequently syringe-infiltrated in the leaves with G3P or the corresponding mock control as indicated below the panel. The treated plants were incubated with Col-0 wt receivers in gas-tight containers. The receiver plants were subsequently released from the containers and inoculated with Pst. The resulting in planta Pst titers at 4 dpi are shown. e Chemical complementation of the SAR-deficient phenotype of the gly1-3 mutant with Pip and G3P. gly1-3 mutant plants were irrigated with Pip or the corresponding H₂O control and subsequently syringe-infiltrated in the first two true leaves with G3P or the corresponding mock control as indicated below the panel. Leaves systemic to the site of G3P application were inoculated with Pst, and the resulting in planta Pst titers at 4 dpi are shown. b–e Dots indicate individual results from two (b, c, e) to three (d) biologically independent experiments, including three replicates each. Bars represent the average of the indicated results ± standard deviation. b Asterisks above bar indicate a significant difference from the mock control (t test, P < 0.001). c–e Different letters above bars indicate significant differences, one-way ANOVA, P < 0.05