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. 2019 Aug 23;5:21. doi: 10.1038/s41522-019-0094-5

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© The Author(s) 2019, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Adhesion and growth of wild-type cells under flow. a Representative dark field images of biofilm formation under flow are shown for wild-type CAI4 + URA cells grown at 23 °C at 2, 5, and 18 h of growth. Arrow indicates direction of flow for every image. b the total biomass within the imaging region (determined by densitometry analysis), the rate of cell attachment, and the biomass detachment (detachment rate normalized to the biomass) over time are shown. Scale bar indicates 50 µm. Data are means of n ≥ 3 independent experiments. Inset shows means ± s.d.