Fig. 4.

The transcription factor knockouts Δefg1 and Δbcr1 show early attachment, but do not remain adherent during growth. a Representative dark field images of biofilm formation under flow are shown for CAI4 + URA, Δefg1, and Δbcr1 cells, as well as CAI4 cells treated with rapamycin (20 nM) at 2, 5, and 18 h of growth at 23 °C. Arrow indicates direction of flow for every image. b the total biomass within the imaging region (determined by densitometry analysis), the rate of cell attachment, and the biomass detachment (detachment rate normalized to the biomass) over time are shown for each strain and condition. Scale bar indicates 50 µm. Data are means of n ≥ 3 independent experiments