Table 2.
The rate of growth over time in Candida albicans biofilms grown under flow
| Early BGRa (×105) | Late BGRa (×105) | Difference in BGR (Late–Early) (×105) | |
|---|---|---|---|
| WT | 2.30 (0.06) | 4.87 (0.37) | 2.57 |
| Δ/Δals1/3 | 1.00 (0.03) | 0.41 (0.05) | −0.59 |
| Δhyr1 | 0.28 (0.02) | 0.15 (0.03) | −0.13 |
| Δhwp2 | 0.05 (0.01) | 0.01 (0.00) | −0.04 |
| Δeap1 | 0.14 (0.02) | 0.00 (0.00) | −0.14 |
| Δihd1 | 0.05 (0.01) | −0.00 (0.00) | −0.05 |
| WT | 2.30 (0.06) | 4.87 (0.37) | 2.57 |
| Δhog1 | 2.31 (0.11)NS | 43.85 (3.36) | 41.54 |
| Δsfl1 | 4.08 (0.11) | 55.09 (0.87) | 51.01 |
| WT | 2.30 (0.06) | 4.87 (0.37) | 2.57 |
| Δbcr1 | 1.94 (0.17)NS | −0.30 (0.04) | −2.24 |
| Δefg1 | 1.72 (0.15) | −0.63 (0.07) | −2.35 |
| Rapamycin | 0.79 (0.04) | 0.68 (0.02) | −0.11 |
| WT | 2.30 (0.06) | 4.87 (0.37) | 2.57 |
| Δywp1 | 4.01 (0.11) | −1.19 (0.20) | −5.20 |
Each value represents a best-fit slope of n ≥ 3 experiments, with standard deviation in parentheses. Early and Late values are determined from data collected between 0 to 5 h and 5 to 18 h, respectively
NS indicates no significant difference between each strain compared to wild-type CAI4 cells. All other values were significant (p < 0.05)
aBiofilm Growth Rate (BGR) was calculated as the best fit slope of the biomass over time in gray values/mm2/h obtained by linear regression