Table 3.

Detachment rate and adhesion maintenance of Candida albicans biofilms under flow

	Early CDRa	Late CDRa	Early AMSb	Early AMS log2 (fold change) over WT	Late AMSc	Late AMS log2 (fold change) over WT
WT	0.18 (0.01)	0.10 (<0.01)	5.45		9.88
Δ/Δals1/3	0.22 (0.01)NS	0.17 (<0.01)	4.58NS	NS	5.97	−0.73
Δhyr1	0.23 (0.01)	0.36 (0.01)	4.33	−0.33	2.81	−1.81
Δhwp2	0.34 (0.02)	0.20 (0.01)	2.98	−0.87	5.08	−0.96
Δeap1	0.27 (0.01)	0.28 (0.01)	3.74	−0.54	3.54	−1.48
Δihd1	0.38 (0.01)	0.13 (<0.01)	2.60	−1.07	7.49	−0.40
WT	0.18 (0.01)	0.10 (<0.01)	5.45		9.88
Δhog1	0.11 (0.01)	0.03 (<0.01)	9.23	0.76	36.76	1.90
Δsfl1	0.04 (<0.01)	0.04 (<0.01)	24.51	2.17	23.20	1.23
WT	0.18 (0.01)	0.10 (<0.01)	5.45		9.88
Δbcr1	0.41 (0.01)	0.21 (<0.01)	2.47	−1.14	4.71	−1.07
Δefg1	0.20 (0.01)NS	0.20 (<0.01)	5.06NS	NS	4.98	−0.99
Rapamycin	0.13 (0.01)	0.12 (<0.01)	7.70	0.50	8.22	−0.27
WT	0.18 (0.01)	0.10 (<0.01)	5.45		9.88
Δywp1	0.10 (<0.01)	0.33 (<0.01)	10.40	0.93	3.06	−1.69

Each value represents a best-fit slope of n ≥ 3 experiments, with standard deviation in parentheses. Data was divided into Early (0–5 h) and Late (5–18 h) phases, as many strains displayed an apparent change in rate around 5 h

NS indicates no significant difference between each strain compared to wild-type CAI4 cells. All other values were significant (p < 0.05)

^aCell Detachment Rate (CDR) was calculated as the average total detachment rate (average biomass of newly detached cells/mm²/h) divided by the biomass of biofilm. Values indicate the average proportion of biomass that detaches from the biofilm per hour

^bAdhesion Maintenance Strength (AMS) was calculated as the inverse of the BDR. Value is reflective of the strength with which a cell remains adhered to the cells or substrate of a biofilm over time