Table 4.

Attachment and detachment of Saccharomyces cerevisiae under flow

	Attachment Ratea (×105)	Early CDR (0–10 h)b	Late CDR (10–18 h)b	Early AMS (0–10 h)c	Late AMS (10–18 h)c
ScWT	5.49 (0.12)	0.25 (0.01)	0.47 (0.04)	3.93	3.62
Sc-CaYWP1	8.89 (0.11)	0.28 (<0.01)NS	0.21 (0.01)	2.14	4.72

Each value represents a mean or best-fit slope of n ≥ 3 experiments, with standard deviation in parentheses

NS indicates no significant difference between each strain compared to wild-type cells. All other values were significant (p < 0.05)

^aAttachment Rate represents the average biomass of newly attached cells /mm²/h within the first 2 h (the attachment phase). Cell detachment does not impact the attachment rate

^bCell Detachment Rate (CDR) was calculated as the average total detachment rate (average biomass of newly detached cells/mm²/h) divided by the biomass of biofilm for the first two hours. Values indicate the average proportion of biomass that detaches from the biofilm per hour

^cAdhesion Maintenance Strength (AMS) was calculated as the inverse of the BDR. Value is reflective of the strength with which a cell remains adhered to the cells or substrate of a biofilm over time