Skip to main content
. 2019 Aug 23;9:12303. doi: 10.1038/s41598-019-48845-3

Table 2.

Summary of the fitting parameters for the peak concentration-response relationship measured for wild-type and mutant hGluN1/hGluN3A receptors expressed in HEK293 cells (see Fig. 6h).

Receptor EC50 (μM)a h a n
hGluN1-4a/hGluN3A 52.0 ± 8.2 1.52 ± 0.23 4
hGluN1-4a/hGluN3A-N549S 103.0 ± 7.9* 0.82 ± 0.04* 5
hGluN1-4a-F484A/hGluN3A 342.2 ± 24.4 1.45 ± 0.07 9
hGluN1-4a-F484A/hGluN3A-N549S 536.4 ± 53.9* 0.92 ± 0.05* 8

aThe EC50 values and Hill coefficient (h) were obtained by fitting the normalized data from each cell using Equation (1). Data are presented as the mean ± SEM, and n corresponds to the number of cells recorded. Statistical analysis was performed for logEC50 and logHill values; *p < 0.05 vs. hGluN1-4a/hGluN3A or hGluN1-4a-F484A/hGluN3A (Student’s t-test).