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. 2019 Aug 19;12:6639–6651. doi: 10.2147/OTT.S208781

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© 2019 Wang et al.

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Identification of GPX2 as a key regulator of intracellular H₂O₂ levels and an inhibitor of apoptosis.

Notes: (A) FACS analysis of ROS levels using DCF-DA probe in ME180 and HeLa cell lines after transfection. (B) Colony formation assay of ME180 and HeLa cells after transfection in the presence or absence of 0.2 mmol/L NAC and 400 µmol/L H₂O₂; the sum of the clone area was calculated by the Image-Pro Plus 6.0. (C) Western blot analysis of the activated caspase-3 protein levels in ME180 and HeLa cells after transfection. Means ± S.D. for three independent experiments are shown. *P<0.05; **P<0.01; ***P<0.001; ns, not significant.