Figure 3.

PKMYT1 is overexpressed in ESCC cell lines, and silenced PKMYT1 contributes to decreased G2/M ratio in cell cycle, inhibits proliferation in ESCC cells and promotes cell apoptosis. (A) The RT-qPCR results indicated that PKMYT1 was up-regulated in ESCC cell lines than normal cell line. (B) PKMYT1 was inhibited by siRNAs. (C) Down-regulated the expression of PKMYT1 in ESCC cells decreased G2/M ratio in cell cycle. (D, E) CCK8 assays (D) and colon assays (E) indicated that down-regulated the expression of PKMYT1 inhibited cell proliferation in ESCC cells.(F) Flow cytometry showed inhibited the expression of PKMYT1 contributed to high apoptosis rate in ESCC cells. (G) Western blotting indicated that the expression of apoptosis-related proteins was changed, the expression of Bcl-2 was decreased but the expression of Bax and cleaved caspase 3 was increased. *P<0.05, **P<0.01, *** P<0.001,****P<0.0001.

Abbreviations: PKMYT1, protein kinase, membrane associated tyrosine/threonine; ESCC, esophageal squamous cell carcinoma; NC, negative control.