Figure 7.
Gln3 phosphorylation levels in glutamine- or proline-grown wild type, ure2Δ, or pph21Δ,pph22Δ double-mutant or pph21Δ,pph22Δ,ure2Δ triple-mutant cells in the presence or absence of rapamycin treatment. Wild type (TB123), and pph21Δ,pph22Δ (03705d), ure2Δ (TB138-1a) and pph21Δ,pph22Δ,ure2Δ (FV165) mutant strains were grown to an A600nm = ∼0.5 in YNB-glutamine (A and B) or YNB-proline (C and D). The untreated cultures were sampled without addition of rapamycin (A and C) or following 20 min incubation with 200 ng/ml rapamycin (+Rap) (B and D). The cultures were then sampled, and the samples processed for western blot analysis.