Figure 8.
Effects of abolishing PP2A when Gln3 is restricted to the nucleus. Wild type (JK9-3da) and pph21Δ,pph22Δ (FV239) were transformed with pRR752 (NES Gln3-Myc13), and grown in ammonia medium in the presence or absence of Msx for 30 min. Sample collection and western blot analysis were as described in Materials and Methods. Upper panel data derived from a six percent acrylamide gel, whereas the lower panel data derived from a parallel sample of the same culture, but electrophoresed for a shorter time in a 7% gel to retain the loading control, Pgk1, within the gel.