Figure 9.

(A–C) Time course of Gln3-Myc¹³ intracellular localization and Gln3-Myc¹³ phosphorylation during short- and long-term nitrogen starvation of pph21Δ,pph22Δ cells followed by refeeding of excess nitrogen (0.1% glutamine final concentration). These experiments were performed, and the data evaluated and presented as described in Figure 5, except that a pph21Δ,pph22Δ mutant (03705d) was used in place of the sit4Δ mutant strain. Data are averages, and SD of four biological replicates for starvation and two for refeeding. (D) A film that was exposed for a shorter period of time to facilitate evaluation of the loading control, Pgk1.