Figure 1.

Southern blot analysis of M. furfur insertional mutants selected in the insertional genetic screen as having a phenotype different from WT. Genomic DNA was digested with SacII, which does not cut within the NAT or NEO cassette, and hybridized with the ORFs of the NEO (left) and NAT (right) genes. Each hybridization band corresponds to a single T-DNA insertion. The names of the transformants and of the M. furfur WT are indicated. NAT, nourseothricin; NEO, G418; T-DNA, transfer DNA; WT, wild-type.