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. 2019 Jun 26;212(4):1163–1179. doi: 10.1534/genetics.119.302329

Figure 2.

Figure 2

T-DNA insertion sites in 15 transformants of M. furfur as determined by iPCR. For each transformant, the mutated region and its corresponding region in the WT are shown. The region altered by the T-DNA is indicated above the sequence. When the sequences of two regions are shown above the sequence, it indicates that the T-DNA insertion involved different locations of the M. furfur genome. The borders of the T-DNA are depicted in bold. Uppercase letters represent nucleotides corresponding to regions with RNAseq coverage (and hence representing either 5ʹ- or 3ʹ-UTRs as indicated, or ORFs), while lowercase letters represent intergenic regions with no RNAseq coverage. The red nucleotides in strain 6A10 indicate a TAG stop codon. The symbol “−/−” indicates chromosomal rearrangement, with genomic locations shown in parentheses for the two transformants (2H11 and 5F10) having rearrangements involving the same chromosome. iPCR, inverse PCR; RNAseq, RNA sequencing; T-DNA, transfer DNA; WT, wild-type.