Table 1. Insertional mutants for M. furfur isolated in the forward genetic screen.
Strains | Phenotype | Hit gene | Position | Comments |
---|---|---|---|---|
1A7 | UV | CDC55 | ORF | Fourth exon |
1F12 | CdSO4 | NA | NA | NA |
2A8 | YNB | ARG1 | ORF | Exon |
2F4 | CdSO4 | SEC13-PRP43 | SEC13-5′ region PRP43-5′ region | Intergenic |
2G9 | FLC | NA | NA | Tandem insertion |
2H11 | FLC, NaCl | ERG5/PDA1 | ERG5-ORF/PDA1-5′ region | Chromosomal rearrangement |
3A1 | CdSO4 | JLP2/TCP1 | JLP2-ORF/TCP1-5′ region | Chromosomal rearrangement |
4A10 | NaNO2, SDS | Uncharacterized enoyl-CoA hydratase | ORF | 2 T-DNA insertions |
4B1 | CdSO4 | MAE1 | 3′-UTR | 2 T-DNA insertions |
5D11 | FLC | NA | NA | Tandem insertion |
5F1 | 37°C | GPD1/INO80 | GPD1-5′ region/INO80-5′ region | Chromosomal rearrangement |
5F10 | NaCl | DUG1/RPC10 | DUG1-3′UTR/RPC10-5′-UTR | Chromosomal rearrangement |
6A10 | FLC | SIP5 | ORF | Altered stop codon |
6B2 | 37°C | Uncharacterized Rho GTPase | ORF | 2 T-DNA insertions |
6C8 | YNB | TYR1 uncharacterized gene | ORF | Nonstandard T-DNA insertion |
7D5 | 37°C | NA | NA | NA |
7D9 | FLC | PDR10- UCB6 | PDR10-5′ region UBC6-3′ region | Intergenic |
7F8 | FLC | ADY2 | 3′-UTR | 260 bp after the predicted stop codon |
7H6 | 37°C | JEN1 uncharacterized protein with RNA-binding domain | JEN1-5′ region uncharacterized 3′ region | Intergenic |
FLC, fluconazole; NA: inverse PCR failed to identify T-DNA insertions; T-DNA, transfer DNA; YNB, yeast nitrogen base; (/), chromosomal rearrangements.