Table 2. Plasmids used in the present study.

Name	Background	Relevant features	Purpose	References
pAIM2	pPZP-201BK	pACT1-NAT-tACT1	Random insertional mutagenesis	Ianiri et al. (2016)
pAIM6	pPZP-201BK	pACT1-NEO-tACT1	Random insertional mutagenesis	Ianiri et al. (2016)
pPZP-201BK	NA	KAN-R	Binary vector that replicates in E. coli and A. tumefaciens	Covert et al. (2001)
pGI3	pPZP-201BK	ScURA3 + 2µ; KAN-R	Binary vector that replicates in E. coli, A. tumefaciens, and S. cerevisiae	Ianiri et al. (2017b)
pGI40	pPZP-201BK	pH3-CAS9-tH3 + p5S rRNA-gRNA CDC55	Cas9 endonuclease and CDC55 target gRNA	This study
pGI41	pGI3	cdc55::NAT	HDR template to generate cdc55Δ	This study
pGI42	pGI3	pdr10::NAT	HDR template to generate pdr10Δ	This study
pGI48	pGI40	pH3-CAS9-tH3 + p5S rRNA-gRNA PDR10	Cas9 endonuclease and PDR10_1 target gRNA	This study

HDR, homology-directed repair; KAN-R, kanamycin-resistant; gRNA, guide RNA.