Table 2. Recombination rates as measured in the inverted repeat reporter assay.
| Genotype | Cβ2/Cβ2 His+ homologous recombination rate (×10−6) | Cβ2/Cβ2-ns His+ divergent recombination rate (×10−6) | Cβ2/Cβ2 rate/Cβ2/Cβ2-ns ratea |
|---|---|---|---|
| Wild-type | 0.52 (0.48–0.56)b | 0.027 (0.024–0.029)b | 20 (17–22)b |
| msh2Δ | 0.92 (0.70–1.15) | 2.55 (2.31–2.80) | 0.36 (0.28–0.47) |
| cac1Δ | 1.19 (1.00–1.38) | 0.030 (0.021–0.040) | 40 (28–57) |
| rtt106Δ | 1.87 (1.55–2.20) | 0.028 (0.018–0.038) | 68 (46–101) |
| asf1Δ | 3.96 (3.30–4.61) | 0.27 (0.22–0.32) | 15 (11–19) |
| rtt109Δ | 3.59 (2.93–4.24) | 0.22 (0.16–0.27) | 16 (12–23) |
| cac1Δ asf1Δ | 0.60 (0.32–0.89) | 0.093 (0.059–0.13) | 6.4 (3.5–12) |
| rtt106Δ asf1Δ | 1.98 (1.65–2.31) | 0.14 (0.097–0.18) | 14 (10–20) |
| cac1Δ rtt106Δ | 2.33 (1.85–2.80) | 0.018 (0.0095–0.027) | 134 (78–229) |
| pol30-8 | 0.74 (0.61–0.87) | 0.026 (0.020–0.033) | 28 (21–39) |
| hst3Δ | 1.67 (1.44–1.89) | 0.090 (0.066–0.11) | 19 (14–25) |
| hst4Δ | 5.31 (4.29–6.34) | 0.36 (0.29–0.43) | 15 (11–19) |
| hst3Δ hst4Δ | 11.3 (8.66–14.8) | 4.53 (3.79–5.42) | 2.5 (1.8–3.5) |
| hst1Δ | 1.34 (1.13–1.55) | 0.047 (0.033–0.063) | 29 (20–41) |
| sir2Δ | 2.12 (1.93–2.31) | 0.050 (0.040–0.059) | 43 (35–53) |
| rpd3Δ | 0.25 (0.22–0.29) | 0.026 (0.020–0.033) | 9.7 (7.4–13) |
| gcn5Δ | 0.98 (0.86–1.10) | 0.068 (0.054–0.082) | 15 (11–19) |
| sir2Δ cac1Δ | 4.44 (3.77–5.11) | 0.030 (0.015–0.046) | 151 (87–264) |
| sir2Δ rtt106Δ | 5.40 (4.36–6.43) | 0.12 (0.089–0.16) | 44 (31–62) |
| sir2Δ cac1Δ rtt106Δ | 6.32 (5.26–7.37) | 0.18 (0.13–0.24) | 35 (25–50) |
| msh2Δ cac1Δ | 3.18 (2.58–3.78) | 5.61 (5.01–6.20) | 0.57 (0.45–0.70) |
| msh2Δ rtt106Δ | 4.07 (3.49–4.65) | 3.35 (2.98–3.67) | 1.2 (1.0–1.4) |
| msh2Δ sir2Δ | 4.87 (4.23–5.50) | 3.58 (3.05–4.11) | 1.4 (1.1–1.7) |
| Wild-type-2μ empty vectorc | 0.74 (0.63–0.85) | 0.034 (0.022–0.048) | 22 (15–33) |
| Wild-type-2μ MSH2-MSH6c | 5.69 (4.82–6.54) | 0.061 (0.043–0.080) | 94 (67–132) |
| sir2Δ-2μ empty vector | 4.35 (3.58–5.13) | 0.067 (0.045–0.089) | 66 (45–96) |
| sir2Δ-2μ MSH2-MSH6 | 4.55 (3.52–5.59) | 0.040 (0.027–0.053) | 116 (77–173) |
| rtt106Δ-2μ empty vector | 2.01 (1.59–2.43) | 0.044 (0.028–0.061) | 46 (30–71) |
| rtt106Δ-2μ MSH2-MSH6 | 2.49 (2.01–2.97) | 0.15 (0.10–0.20) | 17 (12–25) |
| Cβ2/Cβ2-4L rate (×10−6) | Cβ2/Cβ2 rate/Cβ2/Cβ2-4L rate | ||
| Wild-type | 0.057 (0.042–0.072) | 9.2 (7.0–12.1) | |
| cac1Δ | 0.070 (0.048–0.093) | 17 (12–25) | |
| asf1Δ | 0.61 (0.48–0.74) | 6.5 (4.9–8.6) | |
| cac1Δ asf1Δ | 0.25 (0.17–0.32) | 2.4 (1.4–4.3) | |
| sir2Δ | 0.15 (0.12–0.18) | 14 (11–17) |
Recombination rates were calculated as described in the Materials and Methods. The genotypes of the parental strains are shown in Table 1. Cβ2/Cβ2, homologous substrate; Cβ2/Cβ2-4L = 4-nt loop mismatch substrate; Cβ2/Cβ2-ns, base–base mismatch substrate.
a
Homologous rate (Cβ2-Cβ2)/divergent rate for strains with the same genotype or overexpression plasmid.
b
Numbers in parentheses indicate 95% credible intervals.
c
Data obtained from Chakraborty et al. (2018).