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. Author manuscript; available in PMC: 2019 Aug 23.
Published in final edited form as: J Mol Neurosci. 2004;22(1-2):83–92. doi: 10.1385/JMN:22:1-2:83

Fig. 1.

Fig. 1.

Cytofluorimetric analysis. Rabbit polyclonal anti-PAC1 antibodies followed by Alexa 488 goat anti-rabbit IgG (Molecular Probes) were used to detect the presence of PAC1 receptor on FET and HCT116 colon cancer cell lines. Panels A and B show that respectively 83.06%, and 65.28% cells were positive for PAC1 receptor expression, respectively (M2). Untransfected NIH-3T3 wild-type and PAC1-transfected NIH-3T3 cells (HOP) were used as negative and positive controls for PAC1, respectively (C,D). Rabbit IgG was used as a negative control for a specific binding (M1).