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. Author manuscript; available in PMC: 2019 Aug 23.
Published in final edited form as: J Mol Neurosci. 2004;22(1-2):83–92. doi: 10.1385/JMN:22:1-2:83

Fig. 2.

Fig. 2.

Confocal analysis. Rabbit polyclonal anti-PAC1 antibodies followed by Alexa 488 goat anti-rabbit IgG (Molecular Probes) confirmed the presence of PAC1 receptor on FET and HCT116 colon cancer cell lines (A,C). Panels B and D show FET and HCT116 incubated with rabbit IgG followed by Alexa 488-goat anti-rabbit IgG (Molecular Probes). In addition, PAC1-transfected NIH-3T3 and untransfected NIH-3T3 cells were used as PAC1-positive and -negative controls, respectively (E,F).