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. Author manuscript; available in PMC: 2019 Aug 23.
Published in final edited form as: J Mol Neurosci. 2004;22(1-2):83–92. doi: 10.1385/JMN:22:1-2:83

Fig. 3.

Fig. 3.

PCR analysis. PAC1 primers detected the presence of PAC1 receptor mRNAat ~1.35 kb. The primers used were (sense 1) 5′-TGCTGGCCAAGTGTCATG-3′ (nucleotides 50–70) and (antisense) 5′-CTGGGACCGCA GGTGC-3′ (nucleotides 1780–1800) (Pisegna and Wank, 1996) in FET and HCT116 cell lines. The SV1 or HIP PAC1 cDNA was used as a positive control.