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. Author manuscript; available in PMC: 2019 Aug 23.
Published in final edited form as: J Mol Neurosci. 2004;22(1-2):83–92. doi: 10.1385/JMN:22:1-2:83

Fig. 5.

Fig. 5.

PACAP-38-induced Ca2+ to PACAP-38. FET and HCT116 cells were loaded with Fluo-4 AM for the visualization of Ca2+ transport under confocal microscopy. Atime series of a total of 40 images with 20-s intervals in between was taken to monitor the Ca2+activity. Upon activation with 2.5 × 10−7 M PACAP-38 at 200 s (tenth frame), there was no Ca2+ movement observed for both FET and HCT116 cells (A,B) relative to the response obtained from the addition of ionomycin. The addition of 10 μM ionomycin at the thirty-fifth frame of imaging induced a peak that signaled maximum calcium response.