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. Author manuscript; available in PMC: 2019 Aug 23.
Published in final edited form as: J Biol Chem. 2000 Nov 17;275(46):36134–36142. doi: 10.1074/jbc.M004612200

FIG. 3. Scatchard plot analysis of 125I-PACAP-27 binding on membranes of NIH/3T3 cells expressing either the WT or truncated mutant receptor.

FIG. 3.

Scatchard plot analysis using 50 pM of 125I-PACAP on membrane preparations was performed on the indicated WT or truncation mutants (Tr406 or Tr433). Each value represents the mean 6 S.E. of at least two experiments performed in triplicate. Specific binding on membranes for the Null variant (left panel) or Hop variant (right panel) and their mutants were fit to a one-site model.