Skip to main content
. Author manuscript; available in PMC: 2019 Aug 23.
Published in final edited form as: J Biol Chem. 2000 Nov 17;275(46):36134–36142. doi: 10.1074/jbc.M004612200

FIG. 5. Internalization of 125I-PACAP-27 by NIH/3T3 cells stably expressing WT or truncated mutant receptor.

FIG. 5.

NIH/3T3 cells stably expressing the indicated hPAC1 NullWT, NullTr406, and NullTr433 (A) and hPAC1 HopWT, HopTr406, and HopTr433 (B) were incubated with 50 pM of 125I-PACAP-27. At the indicated time intervals, cells were subjected to a 0.5 M KSCN solution. Internalized radioligand is defined as 125I-PACAP-27 that could not be stripped by KSCN and is expressed as a percentage of the total saturable bound 125I-PACAP-27 in control cells, processed in parallel without KSCN exposure. Each value represents the means of at least three experiments performed in triplicate.