| Antibodies |
|
|
| Anti-Biotin |
Thermo |
Cat#31952; RRID:AB_2096845 |
| Anti-Map2 |
Millipore |
Cat#MAB3418; RRID:AB_94856 |
| Anti-NeuN |
Millipore |
Cat#MAB377: RRID: AB_2298772 |
| Anti-Alix |
Millipore |
Cat#ABC40; RRID: AB_10806218 |
| Anti-Synaptotagmin |
Millipore |
Cat#MAB5200; RRID:AB_2255625 |
| Anti-Tau1 (monoclonal TAU46) |
Sigma |
Cat#T9450; RRID:AB_477595 |
| Anti-mTOR (monoclonal 7C10) |
Cell Signaling |
Cat#2983P; RRID:AB_2105622 |
| Anti-GFAP |
Dako |
Cat#GA52461–2; RRID:AB_10013382 |
| Anti-GAPDH (monoclonal GAPDH-71.1) |
Sigma |
Cat#G8795; RRID:AB_1078991 |
| Anti-Munc18 (rabbit) |
Sigma |
Cat#M2694; RRID:AB_477176 |
| ABC-HRP (Vectastain, elite) |
Vector labs |
Cat#LS-J1001–1 |
| Anti-Ras (monoclonal EP1125Y) |
Abcam |
Cat#AB52939; RRID:AB_2121042 |
| KIF5A |
Abcam |
Cat#AB118535
|
| CAMKII (monoclonal 6G9) |
Novus |
Cat#NB100–1983; RRID:AB_10001339 |
| PSMD2 |
Cell Signaling |
Cat#1414S; RRID:AB_2797135 |
| Secondary Anti-Mouse Ig conjugated with HRP |
Bio-Rad |
Cat#172–1011; RRID:AB_11125936 |
| Secondary Anti-rabbit Ig conjugated with HRP |
Bio-Rad |
Cat#172–1019; RRID:AB_11125143 |
| Secondary Anti-goat Ig conjugated with HRP |
Bio-Rad |
Cat#172–1034; RRID:AB_11125144 |
| Secondary Anti-mouse Ig conjugated with Alexa568 |
Life technologies |
Cat#A11004; RRID: AB_2534072 |
| Secondary anti-goat Ig conjugated with Alexa 488 |
Life technologies |
Cat#A11055; RRID:AB_2534102 |
| Secondary anti-rabbit Ig conjugated with Alexa 568 |
Life technologies |
CathA10042; RRID:AB_2534017 |
| STREPTAVIDIN ALEXA488 (NANOFLUOROGOLD) |
Nanoprobes |
Cat#7216–0.5ML; RRID:AB_2315780 |
| STREPTAVIDIN ALEXA488 |
Life technologies |
Cat#S11223; RRID:AB_2315383 |
| Biological Samples |
| Healthy Rat fixed brain and optic nerve tissue. |
Envigo (Harlan) |
N/A |
| Healthy Rat dissected retinas, Optic nerve, LGN, SC and frontal cortex |
Envigo (Harlan) |
N/A |
| Chemicals, Peptides, and Recombinant Proteins |
| EZ-Link NHS-biotin |
Thermo |
Cat#20217 |
| Critical Commercial Assays |
| Fluorescence biotin quantitation kit |
Thermo |
Cat#46610 |
| TSA Biotin system |
Perkin Elmer |
Cat#NEL700A001KT |
| ABC-HRP (Vectastain, elite) |
Vector labs |
Cat#LS-J1001–1 |
| Goldenhance EM |
Nanoprobes |
Cat#2113 |
| Experimental Models: Organisms/Strains |
| Rattus novegicus Strain (Sprague Dawley SD) |
Envigo (Harlam) |
IACUC protocol 08–0082 |
| Software and Algorithms |
| ImageJ |
Schneider et al., 2012 |
https://imagej.nih.gov/ij/ |
