Fig 2. Inhibitory effect of E2 on the TNF-α-induced expression of nuclear NF-κB and iNOS through Nrf2.

(A) WT and Nrf2 KO MEFs were treated with 10 ng/mL TNF-α for the indicated times in the absence or presence of 10 nM E2 for 48 h followed by Western blot analysis with NF-κB antibody. (B) WT and Nrf2 KO MEFs were treated with 10 ng/mL TNF-α for 6 h in in the absence or presence of 10 nM E2 for 48 h and harvested for Western blot analysis to measure expression of iNOS. Lamin B and β-actin were used as internal controls to normalize the expression. Mean with SEM. *, p<0.05 for comparison between two groups.