Fig 7. ravS^R656A suppresses a phenotypic deficiency caused by ravR^ΔEAL in a RavS His⁵⁰⁰-dependent manner.

(a, b) Bacterial virulence. Bacterial strains were inoculated onto plant leaves of Brassica oleracea cv. Zhonggan 11. The lesion length was recorded 10 d after inoculation (n = 30). (c) Production of extracellular polysaccharides (EPS). EPS quantification was calculated as the dry weight of EPS vs. the dry weight of bacterial cells (n = 3). (d, e) Bacterial swimming motility. (d) Bacterial strains were inoculated in NYG plates containing 0.15% agar and grown at 28°C for 28 h. (e) Average diameters of the migration zones were measured (n = 10). (f) Morphology of bacterial flagella. Bacterial flagella were observed by TEM after negative staining. A representative image of each strain is shown. Upper panel: bacterial population profile; lower panel: single bacterium. (g) Ratio of bacterial cells with flagella. For each strain, cells with flagella were counted (n = 100). (h) The average flagellar length of bacterial strains (n = 30). (i) fliC mRNA levels in bacterial strains. The amount of fliC mRNA was measured by qRT-PCR. Amplification of cDNA from tmRNA was used as an internal control. The experiment was repeated three times and the result of a representative is shown. In the figure, standard deviations are shown; asterisk: significant difference, as tested by Student’s t-test (P ≤ 0.05). Three biological replicates were performed.