Fig 2. Agilent microRNA microarray analysis.

(A) Heatmaps of genome-wide miRNA profiles of control and TBI rats with and without treatment with either E2 (upper), JM6 (middle) or PMI-006 (bottom), demonstrated that after TBI, each drug restored miRNA expression profiles to patterns that are similar to sham-injured (no TBI) rats. (B) Dynamic principal component analysis (PCA; left) and hierarchical clustering (right) in Qlucore Omics Explorer showing that injured rats treated with JM6 exhibit miRNA expression patterns that resemble those of sham-injured rats. PCA shows that Naïve, Sham, TBI and TBI+JM6 are all distinguishable one from another.