Fig 1. L929 cell supernatant differentiates porcine peripheral blood monocytes into macrophages in vitro.

Cells cultured for (A) 1 (B) 4 and (C) 7 days following seeding onto plastic 24 and 48 well plate or cover slides were stained using Diff Quick. On day 7, cells are chararcterized by large macrophage-like morphology, forming typical cytoplasmic vacuolar structures and extensive pseudopods. Bar = 100 μm, bars within the insert = 20μm.