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. 2019 Aug 23;14(8):e0221560. doi: 10.1371/journal.pone.0221560

Fig 10. Tulathromycin restores non-opsonized phagocytosis of PRRSV-infected porcine MDMs.

Fig 10

Non-opsonized phagocytic uptake of fluorescently labeled zymosan particles in MDMs treated with HBSS (Control) or with tulathromycin (1mg/mL; TUL) for 1h, un-infected or infected with PRRSV (m.o.i. = 0.5; Virus and TUL+Virus) for 12 hours. Following treatment and infection, MDMs were incubated with fluorescently labeled zymosan particles (10:1 ratio; Zymosan: MDMs) for 1h. (A) Representative laser confocal micrographs of porcine MDMs (red, phagocytosed zymosan particles; blue, nuclei (stained with DAPI); green actin (stained with phalloidin). (B) Percentage of macrophages that phagocytosed at least 1 zymosan particle. (C) Percentage of macrophages that phagocytosed at least 5 zymosan particles. n = 150–300 macrophages/group. Images and histograms are representative of 5 independent experiments. Mean ± SEM. # = P<0.05 vs control; * = P<0.05 vs TUL; Δ = P<0.05 vs virus.