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. Author manuscript; available in PMC: 2020 May 16.
Published in final edited form as: Cell Chem Biol. 2019 Feb 28;26(5):634–644.e3. doi: 10.1016/j.chembiol.2019.02.001

Figure 6-. Anti-PEG antibodies can be detected in rhesus plasma after a single administration of RB006.

Figure 6-

Indirect ELISA was used to measure the level of anti-PEG IgG in plasma before and after RB006 administration, with PEG-BSA coated 96-well plates followed by the addition of plasma for recognition and IgG rhesus monkey specific antibody for detection. Six out of 18 healthy rhesus monkeys tested had significant increases in anti-PEG IgG levels between the pre-aptamer injection samples (white) and post-aptamer injection samples (blue), with four of the animals reaching anti-PEG IgG levels greater than 2 SD from the mean of all pre-injection absorbance values (grey dashed line). The route of administration and dosing of the aptamer as well as the time of sample collection are described in Table 1. The animals selected and their numbers are from the original numerical designation in Figure S1. Data represent the mean ± SEM of technical triplicates. ** indicates p-value <0.01, ***indicates p-value <0.001, using a t-test.