Figure 1.

Experimental design. Fourteen-week-old female C57BL/6J mice were subjected to an experimental autoimmune encephalomyelitis (EAE) protocol along with either a “Prevention” (top timeline) or “Intervention” (bottom timeline) treatment regimen of 30 mg/kg dimethyl fumarate (DMF) or methylcellulose (vehicle) administered twice a day by oral gavage. For the prevention regimen, administration of DMF was initiated the day after mice were immunized with myelin oligodendrocyte glycoprotein (residues 35–55; MOG_35–55), whereas for the interventional regimen, treatment was initiated when motor deficits were observed, and visual acuity decreased by at least 10% from baseline. Once initiated, treatments were given until animals were euthanized. Optokinetic tracking (OKT) responses and motor scores were recorded daily.