Figure 7.

Exercise decreases an inflammatory response observed at the level of the RPE in I307N Rho retinal degeneration. The RPE flatmounts from the mice euthanized 1 week after degeneration was induced were immunostained for Iba-1 (red), ZO-1 (green), and 4′,6-diamidino-2-phenylindole (DAPI) (blue). A–C: Representative 100X fluorescent confocal microscopy images of flatmounts, RPE apical side facing up. A: Flatmounts from the uninduced, inactive mice had little or no Iba-1 immunosignal. B: Flatmounts from the inactive induced mice exhibited heavy Iba-1 staining. C: Flatmounts from the induced but active mice exhibited almost no Iba-1 staining, similar to samples from the uninduced mice. D: High-resolution image of the superior region from the inactive induced group; image taken at 600X magnification. E: High-resolution image of the superior region from the active induced group; image taken at 600X magnification. The size markers represent 30 µm.