Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 1;8:e48051. doi: 10.7554/eLife.48051

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019, Ramaglia et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

PMC Copyright notice

Figure 2. — (a) Overlay of CD3 (green) and CD45 (red) identifies CD3⁺CD45⁺ T cells (solid arrows) and CD3^-CD45⁺ leukocytes other than T cells (dotted arrow). (b) Overlay of CD3 (green) and CD68 (red) identifies CD3⁺CD68^- T cells (solid arrows) and CD3^-CD68⁺microglia/macrophages (dotted arrow). Note that the solid arrows in a and b indicates the same CD3⁺CD45⁺CD68^- T cells. (c) Overlay of κ (white), CD3 (green) and CD68 (red) and (d) overlay of λ (white), CD3 (green) and CD68 (red) identify κ⁺CD3^-CD68^- B cells (arrow head in c) that are λ^-CD3^-CD68^- (arrow head in d) and κ^-CD3^-CD68^- B cells (arrow in c) that are λ⁺CD3^-CD68^- (arrow in d), as expected based on the allelic exclusion of κ and λ. (e) Overlay of CD31 (green), CD68 (red) and Collagen (blue) identifies CD31⁺Collagen⁺CD68^- endothelial cells (arrow head) and CD31^-Collagen^-CD68⁺ microglia/macrophages (arrows). (f, g) Granzyme B⁺ cells (arrows). Images in (a and b) as well as images in c) and d) are from the same areas of an active demyelinating lesion. Image in (e) are from the edge of an active demyelinating lesion. Images in (f and g) are from the center of an active demyelinating lesion.

Figure 2—figure supplement 1. — (a) Overlay of CD3 (green) and CD45 (red) identifies CD3⁺CD45⁺ T cells (solid arrows) and CD3^-CD45⁺ leukocytes other than T cells (dotted arrow). (b) Overlay of CD3 (green) and CD68 (red) identifies CD3⁺CD68^- T cells (solid arrows) and CD3^-CD68⁺microglia/macrophages (dotted arrow). Note that the solid arrows in a and b indicates the same CD3⁺CD45⁺CD68^- T cells. (c) Overlay of κ (white), CD3 (green) and CD68 (red) and (d) overlay of λ (white), CD3 (green) and CD68 (red) identify κ⁺CD3^-CD68^- B cells (arrow head in c) that are λ^-CD3^-CD68^- (arrow head in d) and κ^-CD3^-CD68^- B cells (arrow in c) that are λ⁺CD3^-CD68^- (arrow in d), as expected based on the allelic exclusion of κ and λ. (e) Overlay of CD31 (green), CD68 (red) and Collagen (blue) identifies CD31⁺Collagen⁺CD68^- endothelial cells (arrow head) and CD31^-Collagen^-CD68⁺ microglia/macrophages (arrows). (f, g) Granzyme B⁺ cells (arrows). Images in (a and b) as well as images in c) and d) are from the same areas of an active demyelinating lesion. Image in (e) are from the edge of an active demyelinating lesion. Images in (f and g) are from the center of an active demyelinating lesion.