. 2019 Aug 1;8:e48051. doi: 10.7554/eLife.48051

Table 2. Antibody panels for the identification of functional cell types by IMC.

Functional cell type	Antibody panel
Macrophages and microglia
Macrophages	CD45^highHLA⁺TMEM119^-
Activated macrophages	CD45^highHLA⁺TMEM119^-CD68⁺PLP^-
Demyelinating macrophages	CD45^highHLA⁺TMEM119^-CD68⁺PLP⁺
Microglia	CD45^low/+HLA⁺TMEM119⁺
Activated microglia	CD45^low/+HLA⁺TMEM119⁺CD68⁺PLP^-
Demyelinating microglia	CD45^low/+HLA⁺TMEM119⁺CD68⁺PLP⁺
T Cells
CD8⁺ T cells	CD45⁺CD3⁺CD8α⁺CD4^-
Proliferating CD8⁺ T cells	CD45⁺CD8α⁺CD3⁺CD4^-Ki67⁺
Activated CD8⁺ T cells	CD45⁺CD3⁺CD8α⁺CD4^-NFAT⁺
Activated and proliferating CD8⁺ T cells	CD45⁺CD3⁺CD8α⁺CD4^-NFAT⁺Ki67⁺
‘Chronically activated’ CD8⁺ T cells	CD45⁺CD3⁺CD8α^-CD8⁺CD38⁺HLA⁺
CD4⁺ T cells	CD45⁺CD3⁺CD8α^-CD4⁺
Proliferating CD4⁺ T cells	CD45⁺CD8α^-CD3⁺CD4⁺Ki67⁺
Activated CD4⁺ T cells	CD45⁺CD3⁺CD8α^-CD4⁺NFAT⁺
Activated and proliferating CD4⁺ T cells	CD45⁺CD3⁺CD8α^-CD4⁺NFAT⁺Ki67⁺
‘Chronically activated’ CD4⁺ T cells	CD45⁺CD3⁺CD8α^-CD4⁺CD38⁺HLA⁺
B Cells
Naïve B Cells	CD45⁺Igκ/Igλ⁺IgM⁺CD38^-
IgM memory B cells	CD45⁺Igκ/Igλ⁺IgM⁺CD38⁺
Switched memory B cells	CD45⁺Igκ/Igλ⁺IgM^-CD38⁺