Table 1.

Real-time reverse transcriptase-polymerase chain reaction (RT–PCR) primer sequences.

Species	Primer	Forward (5′–3′)	Reverse (5′–3′)
Human	P-SELECTIN	TGAGCACTGCTTGAAGAAAAAGC	CACGTATTCACATTCTGGCCC
	ICAM-1	GGCCGGCCAGCTTATACAC	TAGACACTTGAGCTCGGGCA
	VCAM-1	TCAGATTGGAGACTCAGTCATGT	ACTCCTCACCTTCCCGCTC
	GAPDH	GGACTCATGACCACAGTCCATGCC	TCAGGGATGACCTTGCCCACAG
Mouse	Claudin 3	AAGCCGAATGGACAAAGAA	CTGGCAAGTAGCTGCAGTG
	Zo-1	AGGACACCAAAGCATGTGAG	GGCATTCCTGCTGGTTACA
	P-selectin	CAGCTAGCTAGCGAGAGGACATGGCTG GCTGCC	CCCAAGCTTGCCGCAATAGCTTCACAG GTTGGCAG
	Icam-1	GTGATGCTCAGGTATCCATCCA	CACAGTTCTCAAAGCACAGCG
	Vcam-1	GTTCCAGCGAGGGTCTACC	AACTCTTGGCAAACATTAGGTGT
	Il-33	CAATCAGGCGACGGTGTGGATGG	TCCGGAGGCGAGACGTCACC
	Mmp-9	GCCCTGGAACTCACACGACA	TTGGAAACTCACACGCCAGAAG
	Il-6	AGCCAGAGTCCTTCAGAGAG	GATGGTCTTGGTCCTTAGCC
	Il-1β	GCAACTGTTCCTGAACTCA	CTCGGAGCCTGTAGTGCAG
	Cd-4	TCCTAGCTGTCACTCAAGGGA	TCAGAGAACTTCCAGGTGAAGA
	Il-13	GCAGCATGGTATGGAGTGTG	TGGCGAAACAGTTGCTTTGT
	β-actin	TCCCTGGAGAAGAGCTATGA	CGATAAAGGAAGGCTGGAA