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. Author manuscript; available in PMC: 2019 Oct 3.
Published in final edited form as: Nature. 2019 Apr 3;568(7751):193–197. doi: 10.1038/s41586-019-1064-z

Figure 2. Mutant mRNA decay is required for transcriptional adaptation.

Figure 2

a, qPCR analysis of hbegfb, vegfab, and vclb mRNA levels in upf1;hbegfa, upf1;vegfaa and upf1;vcla double mutant zebrafish. b, qPCR analysis of Rel mRNA levels after siRNA mediated knockdown of indicated proteins in Rela K.O. cells. c, qPCR analysis of Actg1 mRNA levels after siRNA mediated knockdown of indicated proteins in Actb K.O. cells. d, qPCR analysis of injected hif1ab, epas1a, injected vegfaa, and vegfab RNA levels in 6 hpf wt embryos injected with the indicated RNA. b, c, Scr: Scrambled siRNA control. d, wt or control expression set at 1. a-d, n = 3 biologically independent samples. Error bars, mean, s.d. Two-tailed student’s t-test used to assess P values.