a, Percentage of significantly upregulated (Log2 Fold Change K.O.
> wt and P ≤ 0.05) protein-coding genes
exhibiting sequence similarity with Fermt-2,
Actg1 or Actb and those not exhibiting
sequence similarity. b, qPCR analysis of epas1a
mRNA levels in 6 hpf wt zebrafish injected with uncapped RNA composed solely of
the hif1ab mRNA sequences similar to epas1a or uncapped RNA composed solely of
the hif1ab mRNA sequences not similar to
epas1a. c, d, ChIP-qPCR analysis of Wdr5
(c) and H3K4me3 (d) occupancy near the TSS of
Fermt1, Rel and Actg2 in
Fermt-2, Rela and Actg1
K.O. cells, respectively, compared to wt. Quantification of enrichment shown as
fold-enrichment over IgG control. e, Current putative simplified
model of transcriptional adaptation to mutations. TC: termination codon; DFs:
decay factors; RBPs: RNA binding proteins. b, Control expression
set at 1. b-d, n = 3 (b); 2 (c, d);
biologically independent samples. Error bars, mean, s.d. Two-tailed
student’s t-test used to assess P
values.