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. Author manuscript; available in PMC: 2019 Aug 23.
Published in final edited form as: Biochemistry. 2018 Apr 5;57(35):5210–5217. doi: 10.1021/acs.biochem.7b01275

Table 1.

Kinetic parameters determined for wild-type and variant SufS enzymesa

SufS kcat (min−1) Kcys (μM) kcat/ Kcys
(μM−1min−1)		 KSufE (μM) kcat/ KSufE
(μM−1min−1)
WT 12.1 ± 0.4 50 ± 7 0.24 ± 0.03 0.41 ± 0.05 30 ± 4
R92A 7.4 ± 0.2 95 ± 10 0.08 ± 0.01 0.63 ± 0.09 12 ± 2
E96A 13.8 ± 0.6 95 ± 10 0.14 ± 0.02 2.6 ± 0.3 5.3 ± 0.7
E250A 10.0 ± 0.4 81±9 0.12 ± 0.01 2.4 ± 0.2 4.1 ± 0.4
a

All reactions were performed as described in Materials and Methods.