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. Author manuscript; available in PMC: 2020 Aug 22.
Published in final edited form as: Mol Cell. 2019 Jul 16;75(4):725–740.e6. doi: 10.1016/j.molcel.2019.06.011

Figure 2. Requirements of TNRC6 for AGO binding.

Figure 2.

(A) Electrostatic surfaces of three Trp-binding pockets on the PIWI domain of AG02 (top, PDB ID: 6CBD) and AG04 (bottom).

(B) Residues forming the Trp-binding pockets of AG02 in complex with tryptophans (pink) (top) and apo-AG04 (bottom).

(C-l) Gel filtration analyses of AG02 (blue), AG04 (green), TNRC6B fragment (pink), and a mixture of the TNRC6B fragment and either AG02 or AG04 (black).