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. Author manuscript; available in PMC: 2020 Aug 22.
Published in final edited form as: Mol Cell. 2019 Jul 16;75(4):725–740.e6. doi: 10.1016/j.molcel.2019.06.011

Figure 4. Two water clusters inside AG04-RISC.

Figure 4.

(A) Cross sections of AG04-RISC. Water molecules (cyan) are shown as spheres. The guide RNA (red) is displayed as a stick model.

(B) Conservation of internal water molecules among human AGOs. Water molecules equivalent to W9 and W12 in AG04 have slightly different positions, W9’ and W12’, in AG02, due to N623.

(C) Water molecules in LAKE1 (right) and LAKE2 (left). The simulated annealing Fo-Fc map (blue mesh) is contoured at 3.0 σ around the internal water molecules. See also Figures S5C–D.